Identification of genotypes of Influenza A virus in Malaysia

نویسندگان

  • Rahman MM
  • Wong KK
  • Isahak I
  • Rashid ZZ
  • Alfizah H
چکیده

OBJECTIVE Influenza is considered as an emerging disease until today. The present study was undertaken to determine the prevalent genotypes of Influenza A virus in Malaysia. METHODS Influenza A virus was identified from respiratory specimens by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). Phylogenetic analysis of the identified isolates was performed and genotypes were detected. RESULTS A total number of 505 throat swabs and nasopharyngeal aspirates were examined by rRT-PCR at Universiti Kebangsaan Malaysia Medical Centre (UKMMC) in which 65(12.87%) were positive for influenza A. The identified isolates were successfully genotyped by phylogenetic analysis. The identified influenza A genotypes were: H1N1 (42), H3N2 (20) and H5N1 (3). CONCLUSION The findings indicated that 3 genotypes were circulating in Malaysia during 2011 in which H1N1 was the predominant. RESULTS added new genotype (H5N1) identification record in Malaysia that may be added in data base of WHO and CDC.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype

Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...

متن کامل

Prevalence of Influenza A/H1N1 Virus in North of Iran, 2013 to 2017

Background and purpose: Influenza viruses annually spread around the world in seasonal epidemics. The A/H1N1 virus causes more severe conditions compared to other types of Influenza A. This study is an epidemiological survey of influenza A/H1N1 virus in patients attending health care centers in north of Iran during 2013-2017. Materials and methods: Sampling was done in 3037 patients. Influenza...

متن کامل

Identification of Neuraminidase Gene Mutations of Influenza A/H3N2 Isolates in Mazandaran Province, 2016-2018

 Background and purpose: Influenza is one of the viral infections of the respiratory system, which causes death in high-risk groups every year. The genomic changes of influenza virus make it susceptible to drug resistance, therefore, continuous monitoring of the influenza virus is highly important in order to recognize the efficacy of available drugs. The current study investigated the neuramin...

متن کامل

Multiplex Reverse Transcriptase-PCR Assay for Typing and Subtyping of Influenza A (H5 & H9) Virus in Iran

Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. Methods: A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were directed to t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 30  شماره 

صفحات  -

تاریخ انتشار 2014